METTL7B Polyclonal antibody

• 

  WB analysis using 17001-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17001-1-AP (METTL7B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 17001-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17001-1-AP (METTL7B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

METTL7B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17001-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

methyltransferase like 7B, METTL7B

---

Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

17001-1-AP targets METTL7B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10694

  Product name: Recombinant human METTL7B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 39-244 aa of BC020509

  Sequence: MAVLTPKSNRKMESKKRELFSQIKGLTGASGKVALLELGCGTGANFQFYPPGCRVTCLDPNPHFEKFLTKSMAENRHLQYERFVVAPGEDMRQLADGSMDVVVCTLVLCSVQSPRKVLQEVRRVLRPGGVLFFWEHVAEPYGSWAFMWQQVFEPTWKHIGDGCCLTRETWKDLENAQFSEIQMERQPPPLKWLPVGPHIMGKAVK

• Full Name: methyltransferase like 7B
• Calculated Molecular Weight: 244aa,28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC020509
• Gene Symbol: METTL7B
• Gene ID (NCBI): 196410
• RRID: AB_2142199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UX53
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome.; Authors - Daniel Fox
human	IHC	Front Mol Biosci; Characterization of METTL7B to Evaluate TME and Predict Prognosis by Integrative Analysis of Multi-Omics Data in Glioma; Authors - Xiaochuan Chen
human	WB	Ann Transl Med; METTL7B (methyltransferase-like 7B) identification as a novel biomarker for lung adenocarcinoma.; Authors - Jawad Ali
human,mouse	WB	J Oncol; Downregulation of hsa-miR-30b-3p Inhibits the Oncogenicity of Lung Adenocarcinoma by Targeting the METTL7B Gene.; Authors - Ning Li
mouse	WB	iScience; TWIK-related acid-sensitive K+ channel 2 promotes renal fibrosis by inducing cell-cycle arrest; Authors - Jian Zhang
human	WB	Front Immunol; Heart failure-related genes associated with oxidative stress and the immune landscape in lung cancer; Authors - Ruoshu Duan