METTL8 Polyclonal antibody

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• METTL8 Polyclonal antibody (31173-1-AP)

• 

  WB analysis using 31173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31173-1-AP (METTL8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31173-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using METTL8 antibody (31173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

METTL8 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31173-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

methyltransferase like 8

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Tested Applications

• Positive WB detected in: COLO 205 cells, HCT 116 cells, LoVo cells, SW480 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31173-1-AP targets METTL8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34222

  Product name: Recombinant human METTL8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-77 aa of BC025250

  Sequence: MNMIWRNSISCLRLGKVPHRYQSGYHPVAPLGSRILTDPAKVFEHNMWDHMQWSKEEEAAARKKVKENSAVRVLLEE

• Full Name: methyltransferase like 8
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33~40 kDa
• GenBank Accession Number: BC025250
• Gene Symbol: METTL8
• Gene ID (NCBI): 79828
• RRID: AB_3669881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H825
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL8 (methyltransferase-like protein 8) is an m3C methyltransferase that is best known for its mitochondrial role in installing m3C32 on mt-tRNAThr/Ser(UCN). METTL8 has proved to be a transcriptional target of STAT3 that mediates STAT3's functions in mESCs (PMID: 29706498). METTL8 has multiple splicing isoforms and can undergo sumoylation and associate with nuclear RNA-binding proteins to regulate R-loop formation on ribosomal DNA gene (presumably via its methyltransferase activity on m3C) (PMID: 38744809).