MEX3C Polyclonal antibody

MEX3C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22882-1-AP

Synonyms

RING finger protein 194, RING finger and KH domain-containing protein 2, MEX 3C, EC:2.3.2.27, BM-013

---

Validation Data Gallery

• 

  WB analysis of HeLa using 22882-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22882-1-AP (MEX3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22882-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22882-1-AP (MEX3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22882-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22882-1-AP (MEX3C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22882-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22882-1-AP (MEX3C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 22882-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22882-1-AP (MEX3C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

22882-1-AP targets MEX3C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEX3C fusion protein Ag18931
• Full Name: mex-3 homolog C (C. elegans)
• Calculated Molecular Weight: 659 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC148855
• Gene Symbol: MEX3C
• Gene ID (NCBI): 51320
• RRID: AB_2879183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEX3C(RNA-binding E3 ubiquitin-protein ligase MEX3C) is also named as RKHD2, RNF194 and may be involved in post-transcriptional regulatory mechanisms. MEX3C is necessary for normal postnatal growth and enhances the expression of local bone Igf1 expression(PMID:22927639). This protein is expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. At least three MEX3C protein variants can be expressed by alternative splicing and alternative transcription initiation: MEX3C659AA(containing 659 amino acid residues, about 82 kDa), MEX3C464AA(about 64 kDa) and MEX3C372AA(about 50 kDa)(PMID: 28982131,27053362,22863774).

Publications

Species	Application	Title
mouse	IHC	Gastroenterology; A High-Content Screen Identifies MicroRNAs That Regulate Liver Repopulation After Injury in Mice.; Authors - Adam M Zahm
human	WB,IF	Biol Reprod; Oocyte-Specific Expression of Mouse MEX3C652AA in the Ovary and Its Potential Role in Regulating Maternal Fos mRNA.; Authors - Xue Li