MFF Monoclonal antibody

MFF Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, pig, rat, mouse and More (1)







Cat no : 66527-1-Ig


AD033, C2orf33, MFF, mitochondrial fission factor

Tested Applications

Positive WB detected inpig brain tissue, L02 cells, HepG2 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

66527-1-Ig targets MFF in WB, IF, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MFF fusion protein Ag9990
Full Name mitochondrial fission factor
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC000797
Gene Symbol MFF
Gene ID (NCBI) 56947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.


Product Specific Protocols
WB protocol for MFF antibody 66527-1-IgDownload protocol
IHC protocol for MFF antibody 66527-1-IgDownload protocol
IF protocol for MFF antibody 66527-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Phytother Res

Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow

Authors - Jing-Jing Li

J Adv Res

Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics

Authors - Liuyang Tian


Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis

Authors - Yunfan He


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


JAE HO (Verified Customer) (05-06-2019)

25 ug of PC3 cell lysates were used for western blot.500 ug of cell lysates and 2.5 ug of antibodies were used for IP.Comparing to "17090-1-AP", it looks less titer but it works well for WB and IP.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PC3 cell