|Positive WB detected in||pig brain tissue, L02 cells, HepG2 cells, rat brain tissue, mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66527-1-Ig targets MFF in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.
|Tested Reactivity||Human, pig, rat, mouse|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MFF fusion protein Ag9990|
|Full Name||mitochondrial fission factor|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC000797|
|Gene ID (NCBI)||56947|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
JAE HO (Verified Customer) (05-06-2019)
25 ug of PC3 cell lysates were used for western blot.500 ug of cell lysates and 2.5 ug of antibodies were used for IP.Comparing to "17090-1-AP", it looks less titer but it works well for WB and IP.