Tested Applications
| Positive WB detected in | Jurkat cells, human milk, human placenta tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25951-1-AP targets MFG-E8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13621 Product name: Recombinant human MFGE8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC003610 Sequence: MPRPRLLAALCGALLCAPSLLVALDICSKNPCHNGGLCEEISQEVRGDVFPSYTCTCLKGYAGNHCETKCVEPLGMENGNIANSQIAASSVRVTFLGLQHWVPELARLNRAGMVNAWTPSSNDDNPWIQVNLLRRMWVTGVVTQGASRLASHEYLKAFKVAYSLNGHEFDFIHDVNKKHKEFVGNWNKNAVHVNLFETPVEAQYVRLYPTSCHTACTLRFELLGCELNGCANPLGLKNNSIPDKQITASSSYKTWGLHLFSWNPSYARLDKQGNFNAWVAGSYGNDQWLQIFPGNWDNHSHKKNLFETPILARYVRILPVAWHNRIALRLELLGC Predict reactive species |
| Full Name | milk fat globule-EGF factor 8 protein |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-45 kDa, 51 kDa |
| GenBank Accession Number | BC003610 |
| Gene Symbol | MFGE8 |
| Gene ID (NCBI) | 4240 |
| RRID | AB_3669511 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q08431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as Lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearing apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity, and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-like domains, a mucin-like domain, and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous patterns of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with several diseases (PMID: 27429513).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MFG-E8 antibody 25951-1-AP | Download protocol |
| WB protocol for MFG-E8 antibody 25951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MFGE8 antibody (Cat# 25951-1-AP) has 8 total citations. Citations appear in Cancer Letters, Brain, Behavior, and Immunity, Lipids in Health and Disease, Journal of Ethnopharmacology, Life Sciences, Journal of Cellular and Molecular Medicine, Regenerative Therapy, and Heliyon. Research fields span colitis-associated carcinogenesis, neuroinflammation, steatotic liver disease, heart failure, preeclampsia, renal autophagy, neonatal brain injury, and gastric cancer.
| Species | Application | Title |
|---|---|---|
Cancer Lett Defective m(6)A RNA Modification in Macrophages Exacerbates Inflammation and Promotes Colitis-Associated Carcinogenesis by Impairing Efferocytosis. | ||
Brain Behav Immun LXR-β regulates microglial efferocytosis and neuroinflammation in CPSP via STAT6 activation | ||
Lipids Health Dis Druggable genome-wide Mendelian randomization identifies therapeutic targets for metabolic dysfunction-associated steatotic liver disease | ||
J Ethnopharmacol Xin-shu-bao tablets ameliorates ventricular remodeling against HFrEF via PPARγ/MFGE8 pathway based on MALDI-MSI and Lipidomics | ||
Life Sci BTN3A2 interacted with MFGE8 to alleviate preeclampsia by promoting ferroptosis and inhibiting angiogenesis
| ||
J Cell Mol Med Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals |
Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer confirmed successful use in Western Blot on cerebellum lysates at a 1:1000 dilution, reporting that it works well for this application. Overall, the feedback is positive, indicating reliable performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Andrea (Verified Customer) (08-05-2026) | It works by WB on cerebellum lysates
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