MGAT3 Polyclonal antibody, PBS Only

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• MGAT3 Polyclonal antibody, PBS Only (17869-1-PBS)

• 

  WB analysis of COLO 320 using 17869-1-AP (same clone as 17869-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 17869-1-AP (same clone as 17869-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MGAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17869-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

17869-1-PBS targets MGAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12352

  Product name: Recombinant human MGAT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 184-533 aa of BC075026

  Sequence: VPTVVQYSNLPTKERLVPREVPRRVINAINVNHEFDLLDVRFHELGDVVDAFVVCESNFTAYGEPRPLKFREMLTNGTFEYIRHKVLYVFLDHFPPGGRQDGWIADDYLRTFLTQDGVSRLRNLRPDDVFIIDDADEIPARDGVLFLKLYDGWTEPFAFHMRKSLYGFFWKQPGTLEVVSGCTVDMLQAVYGLDGIRLRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRGLIRTGGWFDGTQQEYPPADPSEHMYAPKYLLKNYDRFHYLLDNPYQEPRSTAAGGWRHRGPEGRPPARGKLDEAEV

• Full Name: mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• Calculated Molecular Weight: 533 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC075026
• Gene Symbol: MGAT3
• Gene ID (NCBI): 4248
• RRID: AB_10597697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q09327
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

In the small intestine, ~75% of triacylglycerol (TG) synthesis occurs via the monoacylglycerol acyltransferase (MGAT) pathway.Three MGAT isoforms (MGAT1, MGAT2 and MGAT3) have been identifed based on their extensive homology to DGAT2. MGAT1 is primarily expressed in the adipose tissue, stomach and kidney but not in the small intestine. MGAT2 and MGAT3 are most highly expressed in the small intestine, suggesting that these two MGAT forms are responsible for the resynthesis of dietary TG that is packaged into chylomicrons.