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ACTL9 Polyclonal antibody, PBS Only (29885-1-PBS)

Filter: AllWBIHCIF-P

WB analysis using 29885-1-AP (same clone as 29885-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 29885-1-AP (same clone as 29885-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 29885-1-AP (same clone as 29885-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29885-1-PBS in a different storage buffer formulation.

ACTL9 Polyclonal antibody, PBS Only

Name: ACTL9 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29885-1-PBS

ACTL9 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 29885-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Actin like protein 9, Actin-like protein 9, ACTL7C, HSD21, hypothetical protein MGC33407

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29885-1-PBS targets ACTL9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag31805 Product name: Recombinant human MGC33407 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-172 aa of BC024028 Sequence: MDASRPKSSESQSSLEAPRPGPNPSPNVVNKPLQRDSPGMVADRLPPKTGAVVIDMGTGTCKVGFAGQASPTYTVATILGCQPKKPATSGQSGLQTFIGEAARVLPELTLVQPLRSGIVVDWDAAELIWRHLLEHDLRVATHDHPLLFSDPPFSPATNREKLVEVAFESLRS Predict reactive species
Full Name	hypothetical protein MGC33407
Observed Molecular Weight	45 kDa
GenBank Accession Number	BC024028
Gene Symbol	ACTL9
Gene ID (NCBI)	284382
RRID	AB_2923617
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TC94
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.