Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, SMMC-7721 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14986-1-AP targets MGLL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6970 Product name: Recombinant human MGLL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC000551 Sequence: METGPEDPSSMPEESSPRRTPQSIPYQDLPHLVNADGQYLFCRYWKPTGTPKALIFVSHGAGEHSGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATAGTASPP Predict reactive species |
| Full Name | monoglyceride lipase |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC000551 |
| Gene Symbol | MGLL |
| Gene ID (NCBI) | 11343 |
| RRID | AB_2143189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A0A0C4DFN3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MGLL antibody 14986-1-AP | Download protocol |
| IP protocol for MGLL antibody 14986-1-AP | Download protocol |
| WB protocol for MGLL antibody 14986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MGLL Polyclonal antibody (Cat# 14986-1-AP) has 19 citations spanning journals including Oncogene, Cell Death & Discovery, Free Radical Biology and Medicine, Cell Reports, J Exp Clin Cancer Res, Phytomedicine, Neuroscience, eLife, and others. Research fields cover lipid metabolism, cancer (renal, prostate, cervical, lung), obesity/NAFLD, endocannabinoid signaling, sleep deprivation, and diabetes.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res TRIM21 attenuates renal carcinoma lipogenesis and malignancy by regulating SREBF1 protein stability | ||
Carbohydr Polym A galacturonan from sea buckthorn (Hippophae rhamnoides L.) berry modulates dyslipidemia by promoting hepatic lipolysis and mitochondrial oxidation | ||
Phytomedicine Atractylodin ameliorates obesity-associated hepatic steatosis by regulating the PLIN2-ATGL/CPT1A axis-mediated lipid droplet-mitochondria interactions. | ||
Cell Death Discov Intermittent hypoxia-induced METTL3 downregulation facilitates MGLL-mediated lipolysis of adipocytes in OSAS
| ||
Oncogene Monoacylglycerol lipase blockades the senescence-associated secretory phenotype by interfering with NF-κB activation and promotes docetaxel efficacy in prostate cancer | ||
Int J Biol Macromol PRRSV Nsp4 induces ATGL protein degradation to promote viral replication and lipid droplet accumulation |
