MGLL Polyclonal antibody

• Featured Product
• KD/KO Validated

MGLL Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14986-1-AP

Synonyms

HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, Monoglyceride lipase

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Validation Data Gallery

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  WB analysis of HepG2 using 14986-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14986-1-AP

  IP result of anti-MGLL (IP:14986-1-AP, 4ug; Detection:14986-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, SMMC-7721 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below

Product Information

14986-1-AP targets MGLL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGLL fusion protein Ag6970
• Full Name: monoglyceride lipase
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000551
• Gene Symbol: MGLL
• Gene ID (NCBI): 11343
• RRID: AB_2143189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).

Publications

Species	Application	Title
mouse	WB	Elife; p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.; Authors - Shuting Xia
human	IHC	Front Oncol; Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma.; Authors - Hao Zhang ; KD Validated
rat	WB	J Physiol Biochem; Sleep deprivation induced fat accumulation in the visceral white adipose tissue by suppressing SIRT1/FOXO1/ATGL pathway activation; Authors - Wei Wang
mouse	WB	Neuroscience; The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice.; Authors - Jinming Zhang
human,rat	WB,IHC	Cell Death Discov; Intermittent hypoxia-induced METTL3 downregulation facilitates MGLL-mediated lipolysis of adipocytes in OSAS; Authors - Xiuji Huang ; KD Validated
human	WB,IHC	J Exp Clin Cancer Res; TRIM21 attenuates renal carcinoma lipogenesis and malignancy by regulating SREBF1 protein stability; Authors - Xintian Chen