Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat IgG2a Recombinant Antibody, PBS Only

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  FC experiment of mouse splenocytes using 65663-1-TR (same clone as 65663-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat IgG2a RecAb (65663-1-TR, Clone: M5/114.15.2) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed. This data was developed using the same antibody clone with 65663-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 65663-1-TR (same clone as 65663-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat IgG2a RecAb (65663-1-TR, Clone: M5/114.15.2) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65663-1-PBS in a different storage buffer formulation.

MHC Class II (I-A/I-E) Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65663-1-PBS

Clone No.M5/114.15.2

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

H2Aa, Aalpha, H 2Aa, H2-Aa, Ia 1

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Product Information

65663-1-PBS targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: histocompatibility 2, class II antigen A, alpha
• GenBank Accession Number: BC031711
• Gene Symbol: MHC Class II
• Gene ID (NCBI): 14960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 antibody is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).