Atlantic Blue™ Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

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  FC experiment of mouse splenocytes using AB-65122

  1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Atlantic Blue™ Anti-Mouse MHC Class II (I-A/I-E) (AB-65122, Clone:M5/114.15.2) or 0.5 ug Isotype Control. Cells were not fixed.

MHC Class II (I-A/I-E) Monoclonal Antibody for FC

Cat No. AB-65122

Clone No.M5/114.15.2

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Synonyms

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

AB-65122 targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Activated C57BL/6 mouse spleen cells

• Full Name: histocompatibility 2, class II antigen A, alpha
• GenBank Accession Number: BC031711
• Gene Symbol: MHC Class II
• Gene ID (NCBI): 14960
• RRID: AB_3084920
• Conjugate: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 404 nm / 458 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P14434
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).