|Positive FC detected in||HeLa cells|
|This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.|
|Sample-dependent, check data in validation data gallery|
65161-1-Ig targets MICA/MICB in WB,FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / Mouse IgG2a|
|Immunogen||Transfected C1R cells expressing MICA|
|Full Name||MHC class I polypeptide-related sequence A|
|Calculated molecular weight||383 aa, 43 kDa|
|GenBank accession number||BC016929|
|Gene ID (NCBI)||4276|
|Purification Method||Purified by protein-A affinity chromatography|
|Storage Buffer||PBS with 0.1% sodium azide.|
|Storage Conditions||Store at 2-8°C.|
Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.