FITC Plus Anti-Human MICA/MICB (6D4)

MICA/MICB Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

6D4

Cat no : FITC-65161

Synonyms

MICA, MICB

---

Validation Data Gallery

• 

  FC experiment of HeLa using FITC-65161

  1X10^6 HeLa cells were surface stained with 5 ul FITC Plus Anti-Human MICA/MICB (FITC-65161, Clone:6D4) or Mouse IgG2a Isotype Control. Cells were not fixed. Live cells were gated.

• 

  FC experiment of HeLa using FITC-65161

  1X10^6 HeLa cells were surface stained with 5 ul FITC Plus Anti-Human MICA/MICB (FITC-65161, Clone:6D4) or Mouse IgG2a Isotype Control. Cells were not fixed. Live cells were gated.

---

Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

FITC-65161 targets MICA/MICB in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transfected C1R cells expressing MICA
• Full Name: MHC class I polypeptide-related sequence A
• Calculated Molecular Weight: 383 aa, 43 kDa
• GenBank Accession Number: BC016929
• Gene Symbol: MICA
• Gene ID (NCBI): 100507436
• RRID: AB_2883809
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.

Publications

Species	Application	Title
	FC	Biosci Microbiota Food Health; Diverse impact of a probiotic strain, Lacticaseibacillus paracasei Shirota, on peripheral mononuclear phagocytic cells in healthy Japanese office workers: a randomized, double-blind, controlled trial; Authors - Tomoaki Naito