Tested Applications
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65161 targets MICA/MICB in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Transfected C1R cells expressing MICA Predict reactive species |
| Full Name | MHC class I polypeptide-related sequence A |
| Calculated Molecular Weight | 383 aa, 43 kDa |
| GenBank Accession Number | BC016929 |
| Gene Symbol | MICA |
| Gene ID (NCBI) | 100507436 |
| RRID | AB_2883809 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | Q29983 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus MICA/MICB antibody FITC-65161 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FITC-65161 is the Anti-Human MICA/MICB (6D4) monoclonal antibody. It has 1 citation, published in Biosci Microbiota Food Health (2022). The study investigated the impact of a probiotic strain on peripheral mononuclear phagocytic cells in healthy Japanese office workers, using flow cytometry. The research field falls under immunology and microbiome science, specifically examining immune cell responses to probiotic intervention.
| Species | Application | Title |
|---|---|---|
Biosci Microbiota Food Health Diverse impact of a probiotic strain, Lacticaseibacillus paracasei Shirota, on peripheral mononuclear phagocytic cells in healthy Japanese office workers: a randomized, double-blind, controlled trial |
