MIG6; ERRFI1 Polyclonal antibody, PBS Only

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• MIG6; ERRFI1 Polyclonal antibody, PBS Only (11630-1-PBS)

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  WB analysis using 11630-1-AP (same clone as 11630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11630-1-AP (same clone as 11630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11630-1-AP (same clone as 11630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11630-1-AP (same clone as 11630-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MIG6; ERRFI1 antibody (11630-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11630-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MIG6; ERRFI1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11630-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

11630-1-PBS targets MIG6; ERRFI1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2202

  Product name: Recombinant human MIG6; ERRFI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 113-462 aa of BC025337

  Sequence: CGFKKLTVNGVCASTPPLTPIKNSPSLFPCAPLCERGSRPLPPLPISEALSLDDTDCEVEFLTSSDTDFLLEDSTLSDFKYDVPGRRSFRGCGQINYAYFDTPAVSAADLSYVSDQNGGVPDPNPPPPQTHRRLRRSHSGPAGSFNKPAIRISNCCIHRASPNSDEDKPEVPPRVPIPPRPVKPDYRRWSAEVTSSTYSDEDRPPKVPPREPLSPSNSRTPSPKSLPSYLNGVMPPTQSFAPDPKYVSSKALQRQNSEGSASKVPCILPIIENGKKVSSTHYYLLPERPPYLDKYEKFFREAEETNGGAQIQPLPADCGISSATEKPDSKTKMDLGGHVKRKHLSYVVSP

• Full Name: ERBB receptor feedback inhibitor 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC025337
• Gene Symbol: MIG6
• Gene ID (NCBI): 54206
• RRID: AB_2101525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJM3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.