MINA Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86760-3-RR (same clone as 86760-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86760-3-RR (MINA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86760-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 86760-3-RR (same clone as 86760-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MINA antibody (86760-3-RR, Clone: 251692F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86760-3-PBS in a different storage buffer formulation.

MINA Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86760-3-PBS

Clone No.251692F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

60S ribosomal protein L27a histidine hydroxylase, Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, EC:1.14.11.79, Histone lysine demethylase MINA, MDIG

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Product Information

86760-3-PBS targets MINA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2838

  Product name: Recombinant human MINA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 160-465 aa of BC014928

  Sequence: SLVGSNVYITPAGSQGLPPHYDDVEVFILQLEGEKHWRLYHPTVPLAREYSVEAEERIGRPVHEFMLKPGDLLYFPRGTIHQADTPAGLAHSTHVTISTYQNNSWGDFLLDTISGLVFDTAKEDVELRTGIPRQLLLQVESTTVATRRLSGFLRTLADRLEGTKELLSSDMKKDFIMHRLPPYSAGDGAELSTPGGKLPRLDSVVRLQFKDHIVLTVLPDQDQSDETQEKMVYIYHSLKNSRETHMMGNEEETEFHGLRFPLSHLDALKQIWNSPAISVKDLKLTTDEEKESLVLSLWTECLIQVV

• Full Name: MYC induced nuclear antigen
• Calculated Molecular Weight: 465 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC014928
• Gene Symbol: MINA
• Gene ID (NCBI): 84864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IUF8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)