MIST1 Polyclonal antibody, PBS Only

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• MIST1 Polyclonal antibody, PBS Only (29853-1-PBS)

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  WB analysis using 29853-1-AP (same clone as 29853-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29853-1-AP (MIST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29853-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 29853-1-AP (same clone as 29853-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29853-1-AP (MIST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29853-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 29853-1-AP (same clone as 29853-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29853-1-AP (MIST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29853-1-PBS in a different storage buffer formulation.

MIST1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29853-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

BHLHA15, bHLHb8, Class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, MIST-1

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Product Information

29853-1-PBS targets MIST1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32096

  Product name: Recombinant human BHLHA15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-189 aa of NM_177455

  Sequence: MKTKNRPPRRRAPVQDTEATPGEGTPDGSLPNPGPEPAKGLRSRPARAAARAPGEGRRRRPGPSGPGGRRDSSIQRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLEGPGPKLYQHYQQQQQVAGGALGATEAQPQGHLQRYSTQIHSFREGT*

• Full Name: basic helix-loop-helix family, member a15
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 20-30 kDa
• GenBank Accession Number: NM_177455
• Gene Symbol: MIST1
• Gene ID (NCBI): 168620
• RRID: AB_2935483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7RTS1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Muscle intestine stomach expression 1 (MIST1), also named BHLHA15, is a secretory cell transcription factor that belongs to the basic helix-loop-helix superfamily. MIST1 is mainly expressed in serous secreted cells and essential for the development and maturation of secretory cells. Secretory cells lacking MIST1 exhibit structural and functional defects, including alterations in cytoskeletal organization and cell polarity and improper cell-cell junction. In healthy pancreata, MIST1 facilitates cell-to-cell communication, promotes acinar cell polarity, controls Ca2+ flux, promotes unfolded protein response (UPR) homeostasis, aids in vesicle assembly, and is required for regulated exocytosis. MIST1 was downregulated in pancreatic cancer tissues compared with normal or adjacent pancreatic tissues and lower MIST1 expression in pancreatic cancer cell lines than normal acinar cells (PMID:29859299).