MLF1IP Polyclonal antibody

• Featured Product
• KD/KO Validated

MLF1IP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13186-1-AP

Synonyms

CENPU, CENP-50, CENP50, CENP U(50), CENP U

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Validation Data Gallery

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  WB analysis using 13186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13186-1-AP

  Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, mouse testis tissue, rat liver tissue
• Positive IHC detected in: human testis tissue, human colon tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13186-1-AP targets MLF1IP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLF1IP fusion protein Ag3858
• Full Name: MLF1 interacting protein
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 46-50 kDa, 60 kDa
• GenBank Accession Number: BC031520
• Gene Symbol: MLF1IP
• Gene ID (NCBI): 79682
• RRID: AB_10642941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.

Publications

Species	Application	Title
human	WB,IHC	Cell Signal; Overexpression of MLF1IP promotes colorectal cancer cell proliferation through BRCA1/AKT/p27 signaling pathway.; Authors - Yuting Xu ; KD Validated