MLKL Recombinant monoclonal antibody

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• MLKL Recombinant monoclonal antibody (87741-1-RR)

• 

  WB analysis using 87741-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87741-1-RR (MLKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MLKL Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87741-1-RR

Clone No.253011B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hMLKL, Mixed lineage kinase domain-like protein

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87741-1-RR targets MLKL in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15188

  Product name: Recombinant human MLKL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-263 aa of BC028141

  Sequence: MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQSLESSSGKSPLEISRFKVKNVKTGSASGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

• Full Name: mixed lineage kinase domain-like
• Calculated Molecular Weight: 471 aa, 54 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC028141
• Gene Symbol: MLKL
• Gene ID (NCBI): 197259
• RRID: AB_3745725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NB16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLKL is a pseudokinase effector of necroptosis that becomes activated through RIPK3-mediated phosphorylation, leading to oligomerization and plasma membrane disruption. By executing necroptotic cell death and promoting release of inflammatory mediators, MLKL plays a central role in innate immunity, inflammatory pathology, and host defense. Dysregulation of MLKL-dependent necroptosis has been linked to inflammatory disorders, infection, and cancer-related immune responses.