MLKL Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.195-12.5 ng/mL

Cat no : MP03218-1

Synonyms

hMLKL, Mixed lineage kinase domain-like protein

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP03218-1

  Sandwich ELISA standard curve of MP03218-1, Human MLKL Recombinant Matched Antibody Pair - PBS only. 87741-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15188. 87741-2-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

• 

  Sandwich ELISA sample test of MP03218-1

  Plasma of six individual healthy human donors was measured. The human MLKL concentration of detected samples was determined to be 2.02 ng/mL with a range of 0.22 - 6.91 ng/mL.

• 

  Sandwich ELISA sample test of MP03218-1

  The mean MLKL concentration was determined to be 6.73 ng/mL in HUVEC cell extract based on a 1.2 mg/mL extract load.

---

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP03218-1 targets MLKL in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.195-12.5 ng/mL (Sandwich ELISA);

Capture antibody: MLKL Recombinant monoclonal antibody, PBS Only (Capture) 87741-1-PBS (253011B11). 100 μg. Concentration 1 mg/ml.

Detection antibody: MLKL Recombinant monoclonal antibody, PBS Only (Detector) 87741-2-PBS (253011H6). 100 μg. Concentration 1 mg/ml.

Alternative MLKL matched antibody pairs: MP51035-1, MP51035-2

Matched pair protein standard: Ag15188

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 87741-1-PBS
• Tested Reactivity: human
• Immunogen: MLKL fusion protein Ag15188
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: mixed lineage kinase domain-like
• GenBank accession number: BC028141
• Gene symbol: MLKL
• Uniprot: Q8NB16
• Gene ID (NCBI): 197259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 87741-2-PBS
• Tested Reactivity: human
• Immunogen: MLKL fusion protein Ag15188
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: mixed lineage kinase domain-like
• GenBank accession number: BC028141
• Gene symbol: MLKL
• Uniprot: Q8NB16
• Gene ID (NCBI): 197259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.