Product Information
87741-1-PBS targets MLKL as part of a matched antibody pair:
MP03218-1: 87741-1-PBS capture and 87741-2-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15188 Product name: Recombinant human MLKL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC028141 Sequence: MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQSLESSSGKSPLEISRFKVKNVKTGSASGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK Predict reactive species |
| Full Name | mixed lineage kinase domain-like |
| Calculated Molecular Weight | 471 aa, 54 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC028141 |
| Gene Symbol | MLKL |
| Gene ID (NCBI) | 197259 |
| RRID | AB_3745725 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NB16 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
MLKL is a pseudokinase effector of necroptosis that becomes activated through RIPK3-mediated phosphorylation, leading to oligomerization and plasma membrane disruption. By executing necroptotic cell death and promoting release of inflammatory mediators, MLKL plays a central role in innate immunity, inflammatory pathology, and host defense. Dysregulation of MLKL-dependent necroptosis has been linked to inflammatory disorders, infection, and cancer-related immune responses.
