MLL5 Polyclonal antibody

MLL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14540-1-AP

Synonyms

NKp44L, Myeloid/lymphoid or mixed-lineage leukemia protein 5, KMT2E, Inactive lysine N-methyltransferase 2E, Inactive histone-lysine N-methyltransferase 2E

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Validation Data Gallery

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  WB analysis of HL-60 using 14540-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 14540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14540-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14540-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of K-562 using 14540-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using MLL5 antibody (14540-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells, Raji cells, mouse retina tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

14540-1-AP targets MLL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLL5 fusion protein Ag6044
• Full Name: myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
• Calculated Molecular Weight: 205 kDa
• Observed Molecular Weight: 100 kDa, 70 kDa
• GenBank Accession Number: BC062583
• Gene Symbol: MLL5
• Gene ID (NCBI): 55904
• RRID: AB_2878065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Mol Cell; SARS-CoV-2 RNA stabilizes host mRNAs to elicit immunopathogenesis; Authors - Hailian Zhao