MLL5 Polyclonal antibody, PBS Only

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• MLL5 Polyclonal antibody, PBS Only (14540-1-PBS)

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  WB analysis of HL-60 using 14540-1-AP (same clone as 14540-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  WB analysis using 14540-1-AP (same clone as 14540-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14540-1-AP (MLL5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14540-1-AP (same clone as 14540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14540-1-AP (same clone as 14540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14540-1-AP (MLL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 14540-1-AP (same clone as 14540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using MLL5 antibody (14540-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14540-1-PBS in a different storage buffer formulation.

MLL5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14540-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

14540-1-PBS targets MLL5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6044

  Product name: Recombinant human MLL5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-354 aa of BC062583

  Sequence: MSIVIPLGVDTAETSYLEMAAGSEPESVEASPVVVEKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSVLISKNEVGIFTTPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPDTYLCERCQPRNLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVSKVNDKRRKKSGEKEQHISKCKKAFREGSRKSSRVKGSAPEIDPSSDGSNFGWETKIKAWMDRYEEANNNQYSEGVQREAQRIALRLGNGNDKKEMNKSDLNTNNLLFKPPVESHIQKNKKILKSAKDLPPDAL

• Full Name: myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
• Calculated Molecular Weight: 205 kDa
• Observed Molecular Weight: 100 kDa, 70 kDa
• GenBank Accession Number: BC062583
• Gene Symbol: MLL5
• Gene ID (NCBI): 55904
• RRID: AB_2878065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZD2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.