MLX Monoclonal antibody

MLX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8D2

Cat no : 67042-1-Ig

Synonyms

bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4

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Validation Data Gallery

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  WB analysis using 67042-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67042-1-Ig (MLX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

• 

  IF Staining of HepG2 using 67042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67042-1-Ig targets MLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLX fusion protein Ag28729
• Full Name: MAX-like protein X
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010689
• Gene Symbol: MLX
• Gene ID (NCBI): 6945
• RRID: AB_2882356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.