MMACHC Polyclonal antibody

MMACHC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21462-1-AP

Synonyms

cblC, MMACHC, RP11 291L19.3



Tested Applications

Positive WB detected inRaji cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21462-1-AP is MMACHC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMACHC fusion protein Ag15001
Full Name methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
Calculated Molecular Weight 282 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC006122
Gene Symbol MMACHC
Gene ID (NCBI) 25974
RRIDAB_10732959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.

Protocols

Product Specific Protocols
WB protocol for MMACHC antibody 21462-1-APDownload protocol
IHC protocol for MMACHC antibody 21462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols