MMACHC Polyclonal antibody

MMACHC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21462-1-AP

Synonyms

cblC, MMACHC, RP11 291L19.3

---

Validation Data Gallery

• 

  WB analysis of Raji using 21462-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21462-1-AP (MMACHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21462-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21462-1-AP (MMACHC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 21462-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 21462-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21462-1-AP (MMACHC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Raji cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21462-1-AP is MMACHC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMACHC fusion protein Ag15001
• Full Name: methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
• Calculated Molecular Weight: 282 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC006122
• Gene Symbol: MMACHC
• Gene ID (NCBI): 25974
• RRID: AB_10732959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMACHC, also named as CblC, is located on chromosome 1 and comprises four coding exons. Human MMACHC catalyzes the reductive decyanation of cyanocobalamin and may serve as a trafficking chaperone for intracellular cobalamin. It also catalyzes the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. NMACHC binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.