MMP2 Recombinant antibody, PBS Only

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  WB analysis of HT-1080 using 81515-5-RR (same clone as 81515-5-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 81515-5-RR (MMP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81515-5-PBS in a different storage buffer formulation.

• 

  WB analysis of U-87 MG using 81515-5-RR (same clone as 81515-5-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 81515-5-RR (MMP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81515-5-PBS in a different storage buffer formulation.

MMP2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 81515-5-PBS

Clone No.250316G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

72 kDa gelatinase, 72 kDa type IV collagenase, CLG4A, EC:3.4.24.24, Gelatinase A

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Product Information

81515-5-PBS targets MMP2 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MMP2 fusion protein Eg1650
• Full Name: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 62-72 kDa
• GenBank Accession Number: BC002576
• Gene Symbol: MMP2
• Gene ID (NCBI): 4313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08253
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).