MMP20 Polyclonal antibody

MMP20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55467-1-AP

Synonyms

Enamel metalloproteinase, Enamelysin, matrix metallopeptidase 20, Matrix metalloproteinase 20, MMP 20, MMP20

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Validation Data Gallery

• 

  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55467-1-AP

  Immunofluorescent analysis of HeLa cells using 55467-1-AP (MMP20 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

55467-1-AP targets MMP20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: matrix metallopeptidase 20
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_004771
• Gene Symbol: MMP20
• Gene ID (NCBI): 9313
• RRID: AB_11182261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP20 belongs to the peptidase M10A family. It degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). MMP20 may play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). This antibody is specific to MMP20.

Publications

Species	Application	Title
human	IF	Int J Mol Sci; Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta.; Authors - Hiroki Yoshioka
mouse	WB	Front Genet; Crucial Roles of microRNA-16-5p and microRNA-27b-3p in Ameloblast Differentiation Through Regulation of Genes Associated With Amelogenesis Imperfecta.; Authors - Akiko Suzuki
rat	WB	Cell Prolif; Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA; Authors - Haoman Niu