MMP20 Polyclonal antibody

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  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55467-1-AP

  Immunofluorescent analysis of HeLa cells using 55467-1-AP (MMP20 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

MMP20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55467-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Enamel metalloproteinase, Enamelysin, matrix metallopeptidase 20, Matrix metalloproteinase 20, MMP 20, MMP20

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

55467-1-AP targets MMP20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: matrix metallopeptidase 20
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_004771
• Gene Symbol: MMP20
• Gene ID (NCBI): 9313
• RRID: AB_11182261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60882
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP20 belongs to the peptidase M10A family. It degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). MMP20 may play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). This antibody is specific to MMP20.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Int J Mol Sci; Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta.; Authors - Hiroki Yoshioka
mouse	WB	Front Genet; Crucial Roles of microRNA-16-5p and microRNA-27b-3p in Ameloblast Differentiation Through Regulation of Genes Associated With Amelogenesis Imperfecta.; Authors - Akiko Suzuki
rat	WB	Cell Prolif; Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA; Authors - Haoman Niu
mouse	IHC	Biochim Biophys Acta Mol Basis Dis; A novel Cdc42-YAP-fibronectin signaling axis regulates ameloblast differentiation during early enamel formation; Authors - Jiayi Zhang
human	WB	J Cell Physiol; KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF-κB axis; Authors - Hangbo Liu