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MMP3 Polyclonal antibody

MMP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17873-1-AP

Synonyms

CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1



Tested Applications

Positive WB detected inA2780 cells, A549 cells, HeLa cells, human skeletal muscle tissue, U-87 MG cells, PC-12 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17873-1-AP targets MMP3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP3 fusion protein Ag12359
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC074869
Gene symbol MMP3
Gene ID (NCBI) 4314
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

Protocols

Product Specific Protocols
WB protocol for MMP3 antibody 17873-1-APDownload protocol
IHC protocol for MMP3 antibody 17873-1-APDownload protocol
IF protocol for MMP3 antibody 17873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Bone Res

FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis.

Authors - Dong Guo
humanWB

Clin Cancer Res

BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.

Authors - Zebing Liu
mouse

Gut Microbes

Gut microbiota regulate tumor metastasis via circRNA/miRNA networks.

Authors - Zhuxian Zhu
mouse, humanWB,IHC

Arthritis Rheum

Suppression of hyaluronan synthesis alleviates inflammatory responses in murine arthritis and in human rheumatoid synovial fibroblasts.

Authors - Yoshioka Yutaka Y
mouseIHC

Aging Cell

1,25-Dihydroxyvitamin D protects against age-related osteoporosis by a novel VDR-Ezh2-p16 signal axis.

Authors - Renlei Yang
humanWB

J Nanobiotechnology

Vasorin-containing small extracellular vesicles retard intervertebral disc degeneration utilizing an injectable thermoresponsive delivery system

Authors - Zhiwei Liao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ana Luísa (Verified Customer) (07-25-2022)

Permeabilization: 0,3% Triton X-100 Blocking 2,5% BSA + 0,1% Triton X-100

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Intervertebral disc cells