MNT Polyclonal antibody, PBS Only

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• MNT Polyclonal antibody, PBS Only (23742-1-PBS)

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  WB analysis of HeLa using 23742-1-AP (same clone as 23742-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23742-1-AP (MNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23742-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23742-1-AP (same clone as 23742-1-PBS)

  WB result of MNT antibody (23742-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MNT transfected HeLa cells. This data was developed using the same antibody clone with 23742-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 23742-1-AP (same clone as 23742-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23742-1-AP (MNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23742-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MNT Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 23742-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

bHLHd3, Class D basic helix-loop-helix protein 3, MAD6, MAX binding protein, Max binding protein MNT

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Product Information

23742-1-PBS targets MNT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20666

  Product name: Recombinant human MNT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-582 aa of BC117563

  Sequence: RAHLKECFETLKRNIPNVDDKKTSNLSVLRTALRYIQSLKRKEKEYEHEMERLAREKIATQQRLAELKHELSQWMDVLEIDRVLRQTGQPEDDQASTSTASEGEDNIDEDMEEDRAGLGPPKLSHRPQPELLKSTLPPPSTTPAPLPPHPHPHPHSVALPPAHLPVQQQQPQQKTPLPAPPPPPAAPAQTLVPAPAHLVATAGGGSTVIAHTATTHASVIQTVNHVLQGPGGKHIAHIAPSAPSPAVQLAPATPPIGHITVHPATLNHVAHLGSQLPLYPQPVAVSHIAHTLSHQQVNGTAGLGPPATVMAKPAVGAQVVHHPQLVGQTVLNPVTMVTMPSFPVSTLKLA

• Full Name: MAX binding protein
• Calculated Molecular Weight: 582 aa, 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC117563
• Gene Symbol: MNT
• Gene ID (NCBI): 4335
• RRID: AB_2879314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q99583
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.