MOF Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86910-3-RR (same clone as 86910-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86910-3-RR (MOF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86910-3-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 86910-3-RR (same clone as 86910-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using MOF antibody (86910-3-RR, Clone: 251926C5 ) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86910-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 86910-3-RR (same clone as 86910-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MOF Recombinant monoclonal antibody (86910-3-RR, Clone:251926C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 86910-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86910-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86910-3-RR against Human MOF were performed. The affinity constant is below 1 pM.

MOF Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 86910-3-PBS

Clone No.251926C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MYST1, EC:2.3.1.-, EC:2.3.1.48, Histone acetyltransferase KAT8, hMOF

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Product Information

86910-3-PBS targets MOF in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4802

  Product name: Recombinant human MOF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-467 aa of BC037773

  Sequence: AVQKNSEKYLSELAEQPERKITRNQKRKHDEINHVQKTYAEMDPTTAALEKEHEAITKVKYVDKIHIGNYEIDAWYFSPFPEDYGKQPKLWLCEYCLKYMKYEKSYRFHLGQCQWRQPPGKEIYRKSNISVYEVDGKDHKIYCQNLCLLAKLFLDHKTLYFDVEPFVFYILTEVDRQGAHIVGYFSKEKESPDGNNVACILTLPPYQRRGYGKFLIAFSYELSKLESTVGSPEKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITGGWGAAVCRGRWGSVSMWTGRSQGLLIAVT

• Full Name: MYST histone acetyltransferase 1
• Calculated Molecular Weight: 467 aa, 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC037773
• Gene Symbol: KAT8/MOF
• Gene ID (NCBI): 84148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H7Z6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994).