Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17638-1-AP targets MON1B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9920 Product name: Recombinant human MON1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 199-547 aa of BC024277 Sequence: STLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFTGL Predict reactive species |
| Full Name | MON1 homolog B (yeast) |
| Calculated Molecular Weight | 547 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC024277 |
| Gene Symbol | MON1B |
| Gene ID (NCBI) | 22879 |
| RRID | AB_2918054 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L1V2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MON1B antibody 17638-1-AP | Download protocol |
| WB protocol for MON1B antibody 17638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MON1B polyclonal antibody (Cat# 17638-1-AP) has 1 citation. It was cited in Cell Research (2016, IF 31.1) in a study investigating how Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b. The research field encompasses endosomal vesicular trafficking and membrane fusion mechanisms. The antibody was applied in WB and IF in human samples.
| Species | Application | Title |
|---|---|---|
Cell Res Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.
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