Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, human liver tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10370-1-AP targets MOV10 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0384 Product name: Recombinant human MOV10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 869-1003 aa of BC002548 Sequence: QGQERSVILISTVRSSQSFVQLDLDFNLGFLKNPKRFNVAVTRAKALLIIVGNPLLLGHDPDWKVFLEFCKENGGYTGCPFPAKLDLQQGQNLLQGLSKLSPSTSGPHSHDYLPQEREGEGGLSLQVEPEWRNEL Predict reactive species |
| Full Name | Mov10, Moloney leukemia virus 10, homolog (mouse) |
| Calculated Molecular Weight | 114 kDa |
| Observed Molecular Weight | 110-115 kDa |
| GenBank Accession Number | BC002548 |
| Gene Symbol | MOV10 |
| Gene ID (NCBI) | 4343 |
| RRID | AB_2297897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCE1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MOV10 antibody 10370-1-AP | Download protocol |
| IHC protocol for MOV10 antibody 10370-1-AP | Download protocol |
| IP protocol for MOV10 antibody 10370-1-AP | Download protocol |
| WB protocol for MOV10 antibody 10370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MOV10 antibody (Cat# 10370-1-AP) has 35 citations published in journals including Nature, Science, Neuron, Nat Commun, Mol Cell, EMBO J, Oncogene, PLoS Pathog, J Biol Chem, and J Virol. Research fields span RNA biology, retrotransposition, viral replication (HIV, hepatitis, influenza), germ cell development, cancer, stress granules, phase separation, and interferon signaling.
| Species | Application | Title |
|---|---|---|
Nat Commun The solution structure of Dead End bound to AU-rich RNA reveals an unusual mode of tandem RRM-RNA recognition required for mRNA regulation | ||
Neuron A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. | ||
Mol Cell MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.
| ||
Mol Cell Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The reviewer used the antibody for Western Blot at a 1:8000 dilution on HEK293 cells and MPMV virions, describing it as working "brilliantly." They noted that a sensitive substrate is required for optimal results. Overall, the feedback indicates strong performance and reliability for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ivana (Verified Customer) (08-28-2026) | The antibody works brilliantly; a sensitive substrate must be used
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