CoraLite®594-conjugated MOV10 Polyclonal antibody

• Featured Product
• KD/KO Validated

MOV10 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10370

Synonyms

DKFZp667O1423, FLJ32791, fSAP113, gb110, KIAA1631, MOV10, Putative helicase MOV 10

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-10370

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 MOV10 antibody (CL594-10370) at dilution of 1:200.

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  FC experiment of HeLa using CL594-10370

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MOV10 (CL594-10370) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10370 targets MOV10 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOV10 fusion protein Ag0384
• Full Name: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 110-115 kDa
• GenBank Accession Number: BC002548
• Gene Symbol: MOV10
• Gene ID (NCBI): 4343
• RRID: AB_2919731
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.