MPP5 Polyclonal antibody, PBS Only

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• MPP5 Polyclonal antibody, PBS Only (17710-1-PBS)

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  WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17710-1-AP (same clone as 17710-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

  IP result of anti-MPP5 (IP:17710-1-AP, 3ug; Detection:17710-1-AP 1:1000) with mouse brain tissue lysate 8000ug. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 17710-1-AP (same clone as 17710-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MPP5 antibody (17710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MPP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 17710-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

17710-1-PBS targets MPP5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12010

  Product name: Recombinant human PALS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-341 aa of BC095485

  Sequence: MTTSHMNGHVTEESDSEVKNVDLASPEEHQKHREMAVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPMFDTEEGIVLESPHYAVKILEIEDLFSSLKHIQHTLVDSQSQEDISLLLQLVQNKDFQNAFKIHNAITVHMNKASPPFPLISNAQDLAQEVQTVLKPVHHKEGQELTALLNTPHIQALLLAHDKVAEQEMQLEPITDERVYESIGQYGGETVKIVRIEKARDIPLGATVRNEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPP

• Full Name: membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
• Calculated Molecular Weight: 675 aa, 77 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC095485
• Gene Symbol: MPP5
• Gene ID (NCBI): 64398
• RRID: AB_2282012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3R9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.