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MPV17 Polyclonal antibody
MPV17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 10310-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IF | See 3 publications below |
Product Information
10310-1-AP targets MPV17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MPV17 fusion protein Ag0312 |
| Full Name | MpV17 mitochondrial inner membrane protein |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC001115 |
| Gene Symbol | MPV17 |
| Gene ID (NCBI) | 4358 |
| RRID | AB_2144642 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MPV17, also termed as SYM1, encodes an inner mitochondrial membrane protein. It's potential candidate that may account for the mitochondrial (mt) DNA depletion syndromes (MDDS) characterized by a severe, tissue-specific decrease of mtDNA copy number and ultimate organ failure. It's a human ortholog of the mouse kidney disease gene Mpv17. Absence or malfunction of MPV17 causes oxidative phosphorylation (OXPHOS) failure and mtDNA depletion, not only in affected individuals but also in Mpv17-/- mice. MPV17 contribute to ROS homeostasis, but the downstream signaling is unknown yet. Catalog# 10310-1-AP is a rabbit polyclonal antibody raised against the full-length of human MPV17.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPV17 antibody 10310-1-AP | Download protocol |
| IHC protocol for MPV17 antibody 10310-1-AP | Download protocol |
| IF protocol for MPV17 antibody 10310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet MPV17 encodes an inner mitochondrial membrane protein and is mutated in infantile hepatic mitochondrial DNA depletion.
| ||
Nucleic Acids Res Aberrant ribonucleotide incorporation and multiple deletions in mitochondrial DNA of the murine MPV17 disease model. | ||
Mol Ther AAV-mediated liver-specific MPV17 expression restores mtDNA levels and prevents diet-induced liver failure.
| ||
PLoS Genet MPV17 Loss Causes Deoxynucleotide Insufficiency and Slow DNA Replication in Mitochondria.
| ||
