Tested Applications
| Positive WB detected in | mouse spleen tissue, THP-1 cells, rat spleen tissue |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:250-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13260-1-AP targets MR1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3849 Product name: Recombinant human MR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-278 aa of BC012485 Sequence: SDSRTHSLRYFRLGVSDPIHGVPEFISVGYVDSHPITTYDSVTRQKEPRAPWMAENLAPDHWERYTQLLRGWQQMFKVELKRLQRHYNHSDNVAHTIKQAWEANQHELLYQKNWLEEECIAWLKRFLEYGKDTLQRTEPPLVRVNRKETFPGVTALFCKAHGFYPPEIYMTWMKNGEEIVQEIDYGDILPSGDGTYQAWASIELDPQSSNLYSCHVEHCGVHMVLQVPQESETIPLVMKAVSGSIVLVIVLAGVGVLVW Predict reactive species |
| Full Name | major histocompatibility complex, class I-related |
| Calculated Molecular Weight | 341 aa, 39 kDa |
| Observed Molecular Weight | 39-50 kDa |
| GenBank Accession Number | BC012485 |
| Gene Symbol | MR1 |
| Gene ID (NCBI) | 3140 |
| RRID | AB_2877930 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q95460 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MR1 antibody 13260-1-AP | Download protocol |
| WB protocol for MR1 antibody 13260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MR1 Polyclonal antibody (Cat# 13260-1-AP) has 9 citations published in journals including PNAS, Journal of Biological Chemistry, Cancer Immunology Research, Carbohydrate Polymers, International Immunopharmacology, American Journal of Pathology, Pigment Cell & Melanoma Research, and bioRxiv. Associated research fields span tumor immunotherapy, MR1-mediated antigen presentation, airway inflammation in asthma, primary sclerosing cholangitis, and vitiligo.
| Species | Application | Title |
|---|---|---|
Carbohydr Polym Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy. | ||
Cancer Immunol Res MR1 gene and protein expression are enhanced by inhibition of the extracellular signal-regulated kinase ERK | ||
Int Immunopharmacol Opposite effects of sevoflurane at high and low concentration on the airway inflammation in juvenile asthma rats: A TMT-based proteomics analysis study | ||
Am J Pathol Bile from Patients with Primary Sclerosing Cholangitis Contains Mucosal-Associated Invariant T-Cell Antigens. | ||
J Biol Chem The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation. |
Reviews
What customers say
The single review reports a successful Western Blot using a 1:250 dilution with 80 µg total protein, a milk-blocked PVDF membrane, and an HRP goat anti-rabbit secondary antibody with high-sensitivity ECL detection. The antibody solution could be stored at -20°C and reused multiple times. It was tested on K562 cell lines and primary liver cells, receiving a 4-star rating. Overall, the user found the product reliable and cost-effective for their WB workflow.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tina (Verified Customer) (08-31-2020) | - PAGE was loaded with 80ug total protein - PVDF membrane blocked with milk - first AB dilution 1:250 (stored at -20°C, I could reuse the solution multiple times) - secondary AB I used HRP goat anti-rabbit and a high sensitive ECl to develop the Blot.
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