MRP4 Monoclonal antibody

MRP4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1B10B4

Cat no : 67230-1-Ig

Synonyms

ABCC4, MOAT B, MOATB, MRP4

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Validation Data Gallery

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  WB analysis using 67230-1-Ig

  Non-boil and boiled HepG2, A549, and PC-12 cell lysates were subjected to SDS PAGE followed by western blot with 67230-1-Ig (ABCC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 67230-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67230-1-Ig (ABCC4 antibody) at dilution of 1:1000 (under 40x lens)..

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

67230-1-Ig targets MRP4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MRP4 fusion protein Ag10171
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 4
• Calculated Molecular Weight: 859 aa, 97 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC041560
• Gene Symbol: MRP4
• Gene ID (NCBI): 10257
• RRID: AB_2882517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRP4 (also known as ABCC4), belonging to ATP-binding cassette (ABC) transporter family, is a multi-transmembrane protein that is able to transport a range of organic anionic compounds (both endogenous and xenobiotic) out of the cell. It can exclude various drugs thus is considered as potential target to prevent drug-resistance.

Publications

Species	Application	Title
human	IHC	Front Genet; Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma.; Authors - Xin Zhou
human	WB	J Cell Mol Med; Overcoming flumatinib resistance in chronic myeloid leukaemia: Insights into cellular mechanisms and ivermectin's therapeutic potential; Authors - Jixian Huang