MRP4 Monoclonal antibody

MRP4 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10B4

Cat no : 67230-1-Ig

Synonyms

ABCC4, MOAT B, MOATB, MRP4



Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

67230-1-Ig targets MRP4 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MRP4 fusion protein Ag10171
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 4
Calculated molecular weight 859 aa, 97 kDa
Observed molecular weight 160-180 kDa
GenBank accession numberBC041560
Gene symbol ABCC4
Gene ID (NCBI) 10257
RRIDAB_2882517
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRP4 (also known as ABCC4), belonging to ATP-binding cassette (ABC) transporter family, is a multi-transmembrane protein that is able to transport a range of organic anionic compounds (both endogenous and xenobiotic) out of the cell. It can exclude various drugs thus is considered as potential target to prevent drug-resistance.

Protocols

Product Specific Protocols
WB protocol for MRP4 antibody 67230-1-IgDownload protocol
IHC protocol for MRP4 antibody 67230-1-IgDownload protocol
FC protocol for MRP4 antibody 67230-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma.

Authors - Xin Zhou