MRPL1 Polyclonal antibody

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  WB analysis using 16254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16254-1-AP (MRPL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16254-1-AP (MRPL1 Antibody) at dilution of 1:50 (under 40x lens).

MRPL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16254-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MRP-L1, MRP L1, Large ribosomal subunit protein uL1m, L1mt, BM-022

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16254-1-AP targets MRPL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9270

  Product name: Recombinant human MRPL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-303 aa of BC015109

  Sequence: MVYQTSLCSCSVNIRVPNRHFAAAKKSAKKTKKGAKEKTPDEKKDEIEKIKAYPYMEGEPEDDVYLKRLYPRQIYEVEKAVHLLKKFQILDFTSPKQSVYLDLTLDMALGKKKNVEPFTSVLSLPYPFASEINKVAVFTENASEVKIAEENGAASAGGTSLIQKIWDDEIVADFYVAVPEIMPELNRLRKKLNKKYPKLSRNSIGRDIPKMLELFKNGHEIKVDEERENFLQTKIATLDMSSDQIAANLQAVINEVCRHRPLNLGPFVVRAFLRSSTSEGLLLKIDPLLPKEVKNEESEKEDA

• Full Name: mitochondrial ribosomal protein L1
• Calculated Molecular Weight: 303 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC015109
• Gene Symbol: MRPL1
• Gene ID (NCBI): 65008
• RRID: AB_2145582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BYD6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
human	WB	Cell Rep; A microscopy-based screen identifies cellular kinases modulating mitochondrial translation; Authors - Roya Yousefi