Tested Applications
| Positive WB detected in | BxPC-3 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15543-1-AP targets MRPL11 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7899 Product name: Recombinant human MRPL11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC005002 Sequence: MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKEADLAAQEEAAKK Predict reactive species |
| Full Name | mitochondrial ribosomal protein L11 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC005002 |
| Gene Symbol | MRPL11 |
| Gene ID (NCBI) | 65003 |
| RRID | AB_2297856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3B7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MRPL11 is one of the protein components of mitochondrial ribosomes encoded by the nuclear genome, which is essential for oxidative phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| WB protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPL11 Polyclonal antibody (Cat# 15543-1-AP) has 22 citations. Publications appear in high-impact journals including Science, Nature Medicine, Nature Communications, Nature Structural & Molecular Biology, Signal Transduction and Targeted Therapy, Cell Death & Differentiation, Science Advances, Human Molecular Genetics, American Journal of Human Genetics, Journal of Cell Biology, iScience, and bioRxiv. Research fields span mitochondrial translation, ribosome biogenesis, oxidative phosphorylation, protein synthesis quality control, mitochondrial diseases, cancer, obesity, and neurodegeneration.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes | ||
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
J Exp Clin Cancer Res UBASH3B-mediated MRPL12 Y60 dephosphorylation inhibits LUAD development by driving mitochondrial metabolism reprogramming | ||
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer reported that the antibody "worked on blot," indicating satisfactory performance in their experiment. No specific dilution or tissue/cell type details were provided. Overall, the feedback is positive, confirming the product's reliability for Western Blot use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nathalie (Verified Customer) (09-26-2025) | Worked on blot
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