MRPL11 Recombinant monoclonal antibody

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  IF Staining of HepG2 using 85109-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 85109-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 85109-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MRPL11 Recombinant antibody (85109-4-RR, Clone:242768G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 85109-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85109-4-RR against Human MRPL11 were performed. The affinity constant is 14.5 nM.

MRPL11 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 85109-4-RR

Clone No.242768G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

242768G1, CGI 113, CGI-113, L11mt, Large ribosomal subunit protein uL11m

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85109-4-RR targets MRPL11 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7899

  Product name: Recombinant human MRPL11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-192 aa of BC005002

  Sequence: MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKEADLAAQEEAAKK

• Full Name: mitochondrial ribosomal protein L11
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC005002
• Gene Symbol: MRPL11
• Gene ID (NCBI): 65003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y3B7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.