MRPL12 Recombinant antibody

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  WB analysis using 85070-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85070-5-RR (MRPL12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85070-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (85070-5-RR, Clone: 242622D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85070-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85070-5-RR against Human MRPL12 were performed. The affinity constant is below 1 pM.

MRPL12 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85070-5-RR

Clone No.242622D12

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

242622D12, 5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7

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Tested Applications

• Positive WB detected in: COLO 320 cells, A431 cells, U-251 cells, rat colon tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85070-5-RR targets MRPL12 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MRPL12 fusion protein Ag6504
• Full Name: mitochondrial ribosomal protein L12
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC002344
• Gene Symbol: MRPL12
• Gene ID (NCBI): 6182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P52815
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression.