MRPL15 Polyclonal antibody

• 

  WB analysis using 18339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18339-1-AP (MRPL15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

MRPL15 Polyclonal Antibody for WB, ELISA

Cat No. 18339-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HSPC145, L15mt, MRP L15, MRP L7, MRPL15, RPML7

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

18339-1-AP targets MRPL15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13087

  Product name: Recombinant human MRPL15 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-296 aa of BC000891

  Sequence: MAGPLQGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGRVDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYDPRSLDIVCKPVPFFLRGQPIPKRMLPPEELVPYYTDAKNRGYLADPAKFPEARLELARKYGYILPDITKDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS

• Full Name: mitochondrial ribosomal protein L15
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC000891
• Gene Symbol: MRPL15
• Gene ID (NCBI): 29088
• RRID: AB_2297866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P015
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
mouse	WB	Cell Rep; Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.; Authors - Christopher Lowden
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia