MRPL16 Recombinant monoclonal antibody

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  WB analysis using 83888-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83888-1-RR (MRPL16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83888-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83888-1-RR against Human MRPL16 were performed. The affinity constant is below 1 pM.

MRPL16 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83888-1-RR

Clone No.241069A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PNAS 111, MRP-L16, MRP L16, Large ribosomal subunit protein uL16m, L16mt

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, COLO 320 cells, mouse kidney tissue, mouse brain tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83888-1-RR targets MRPL16 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6523

  Product name: Recombinant human MRPL16 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-251 aa of BC001040

  Sequence: MWRLLARASAPLLRVPLSDSWALLPASAGVKTLLPVPSFEDVSIPEKPKLRFIERAPLVPKVRREPKNLSDIRGPSTEATEFTEGNFAILALGGGYLHWGHFEMMRLTINRSMDPKNMFAIWRVPAPFKPITRKSVGHRMGGGKGAIDHYVTPVKAGRLVVEMGGRCEFEEVQGFLDQVAHKLPFAAKAVSRGTLEKMRKDQEERERNNQNPWTFERIATANMLGIRKVLSPYDLTHKGKYWGKFYMPKRV

• Full Name: mitochondrial ribosomal protein L16
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001040
• Gene Symbol: MRPL16
• Gene ID (NCBI): 54948
• RRID: AB_3671471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NX20
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL16 (Mitochondrial Ribosomal Protein L16) is a protein coding gene. It encodes a 39S subunit protein. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. The molecular mass of MRPL16 is 28 kDa.