MRPL19 Polyclonal antibody

MRPL19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16517-1-AP

Synonyms

MRP L19, MRP L15, Large ribosomal subunit protein bL19m, L19mt, L15mt

---

Validation Data Gallery

• 

  WB analysis of mouse kidney using 16517-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16517-1-AP (MRPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16517-1-AP (MRPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16517-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16517-1-AP (MRPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 16517-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16517-1-AP (MRPL19 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16517-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16517-1-AP (MRPL19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells, mouse liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

16517-1-AP targets MRPL19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL19 fusion protein Ag9738
• Full Name: mitochondrial ribosomal protein L19
• Calculated Molecular Weight: 292 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC030144
• Gene Symbol: MRPL19
• Gene ID (NCBI): 9801
• RRID: AB_2250806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Transl Lung Cancer Res; Overexpression of MRPL19 in predicting poor prognosis and promoting the development of lung adenocarcinoma; Authors - Dong Wei