MRPL28 Polyclonal antibody

MRPL28 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21604-1-AP

Synonyms

L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15

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Validation Data Gallery

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  WB analysis using 21604-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21604-1-AP (MRPL28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21604-1-AP

  IP result of anti-MRPL28 (IP:21604-1-AP, 4ug; Detection:21604-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 21604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 21604-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 21604-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21604-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL28 antibody (21604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, JAR cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 21604-1-AP is MRPL28 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL28 fusion protein Ag16224
• Full Name: mitochondrial ribosomal protein L28
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC000990
• Gene Symbol: MRPL28
• Gene ID (NCBI): 10573
• RRID: AB_10859785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease; Authors - Sumudu S C Amarasekera
human	WB	J Ethnopharmacol; Elucidating the pharmacological effects of Compound Kushen injection on MYC-P15-CCND1 signaling pathway in nasopharyngeal carcinoma - An in vitro study; Authors - Zhishan Wu