MRPL4 Polyclonal antibody

• Featured Product
• KD/KO Validated

MRPL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27484-1-AP

Synonyms

MRP L4, Large ribosomal subunit protein uL4m, L4mt, CGI-28, CGI 28

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Validation Data Gallery

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  WB analysis of HL-60 using 27484-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27484-1-AP (MRPL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27484-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27484-1-AP (MRPL4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27484-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27484-1-AP (MRPL4 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 27484-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL4 antibody (27484-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:40000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

27484-1-AP targets MRPL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL4 fusion protein Ag26568
• Full Name: mitochondrial ribosomal protein L4
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000756
• Gene Symbol: MRPL4
• Gene ID (NCBI): 51073
• RRID: AB_2810294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Metab; Tetracyclines promote survival and fitness in mitochondrial disease models.; Authors - Elizabeth A Perry ; KO Validated
human	WB	Nucleic Acids Res; CoLoC-seq probes the global topology of organelle transcriptomes; Authors - Damien Jeandard
human	WB	bioRxiv; Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival; Authors - Conor T Ronayne
human	WB	EMBO Rep; Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival; Authors - Conor T Ronayne
human	IHC	Sci Rep; Construction of molecular subtype and prognostic model for gastric cancer based on nucleus-encoded mitochondrial genes; Authors - Xu Wang