MRPL44 Polyclonal antibody

MRPL44 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

38

Cat no : 16394-1-AP

Synonyms

L44MT, MRP L44, MRPL44

---

Validation Data Gallery

• 

  WB analysis of rat brain using 16394-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16394-1-AP (MRPL44 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16394-1-AP (MRPL44 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 16394-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16394-1-AP (MRPL44 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 16394-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16394-1-AP (MRPL44 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: A549 cells, rat brain tissue, HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 36 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

16394-1-AP targets MRPL44 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey, zebrafish, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL44 fusion protein Ag9485
• Full Name: mitochondrial ribosomal protein L44
• Calculated Molecular Weight: 332 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC012058
• Gene Symbol: MRPL44
• Gene ID (NCBI): 65080
• RRID: AB_2146062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRP-L44 (mitochondrial ribosomal protein L44) is a 332 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis.

Publications

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia

Related Products

MRPL19 Polyclonal antibody

16517-1-AP

MRPL13 Polyclonal antibody

16241-1-AP

MRPL38 Polyclonal antibody

15913-1-AP

MRPL24 Polyclonal antibody

16224-1-AP

MRPL47 Polyclonal antibody

24728-1-AP

MRPL37 Polyclonal antibody

15190-1-AP

TSR1 Polyclonal antibody

16887-1-AP