Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, SKOV-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15682-1-AP targets MRPL45 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8184 Product name: Recombinant human MRPL45 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 86-329 aa of BC006235 Sequence: AGLVIPPEKSDRSIHLACTAGIFDAYVPPEGDARISSLSKEGLIERTERMKKTMASQVSIRRIKDYDANFKIKDFPEKAKDIFIEAHLCLNNSDHDRLHTLVTEHCFPDMTWDIKYKTVRWSFVESLEPSHVVQVRCSSMMNQGNVYGQITVRMHTRQTLAIYDRFGRLMYGQEDVPKDVLEYVVFEKQLTNPYGSWRMHTKIVPPWAPPKQPILKTVMIPGPQLKPEEEYEEAQGEAQKPQLA Predict reactive species |
| Full Name | mitochondrial ribosomal protein L45 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC006235 |
| Gene Symbol | MRPL45 |
| Gene ID (NCBI) | 84311 |
| RRID | AB_2146065 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| IHC protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| IP protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| WB protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MRPL45 Polyclonal antibody (Cat# 15682-1-AP) has 20 citations across 16 journals, including Cell, Nature Communications, Molecular Cell, Nucleic Acids Research, Science Advances, Nature Structural & Molecular Biology, PNAS, Cell Reports, Cell & Molecular Life Sciences, Journal of Cell Biology, Journal of Cell Science, FEBS Journal, Journal of Biological Chemistry, Disease Models & Mechanisms, Human Molecular Genetics, and Human Mutation. Research fields center on mitochondrial ribosome assembly, mitoribosomal biogenesis, mitochondrial translation, and mitochondrial disease.
| Species | Application | Title |
|---|---|---|
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Nucleic Acids Res Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation | ||
Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. |
Reviews
What customers say
The single review for this product is a 5-star rating from an immunofluorescence experiment. The user performed IF on 4% PFA-fixed BJ-5ta cells, staining for TOM20 (magenta) and MRPL45 (cyan) at a 1:1000 dilution, and included an image showing the results. Overall, the feedback is positive, indicating the antibody performed well in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yan (Verified Customer) (11-03-2025) | 4% PFA fixed BJ-5ta cells immunofluorescence of TOM20 (Magenta) and MRPL45 (Cyan)
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