MRPL45 Polyclonal antibody

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• KD/KO Validated

MRPL45 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 15682-1-AP

Synonyms

L45mt, MRPL45, MRP-L45

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Validation Data Gallery

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  WB analysis using 15682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15682-1-AP

  WB result of MRPL45 antibody (15682-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells.

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  WB analysis of mouse liver using 15682-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15682-1-AP

  IP result of anti-MRPL45 (IP:15682-1-AP, 4ug; Detection:15682-1-AP 1:1000) with HeLa cells lysate 680 ug.

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  IHC staining of human ovary tumor using 15682-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15682-1-AP (MRPL45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15682-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15682-1-AP (MRPL45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15682-1-AP

  Immunofluorescent analysis of HeLa cells using 15682-1-AP (MRPL45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, SKOV-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IF: See 2 publications below

Product Information

15682-1-AP targets MRPL45 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL45 fusion protein Ag8184
• Full Name: mitochondrial ribosomal protein L45
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC006235
• Gene Symbol: MRPL45
• Gene ID (NCBI): 84311
• RRID: AB_2146065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.

Publications

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
human	IF	Proc Natl Acad Sci U S A; Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.; Authors - Gokhan Akman
human	WB	J Cell Biol; FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.; Authors - Liang Wang