Tested Applications
| Positive WB detected in | BxPC-3 cells, A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15542-1-AP targets MRPL49 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7898 Product name: Recombinant human MRPL49 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC004378 Sequence: MAATMFRATLRGWRTGVQRGCGLRLLSQTQGPPDYPRFVESVDEYQFVERLLPATRIPDPPKHEHYPTPSGWQPPRDPPPNLPYFVRRSRMHNIPVYKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLEKGF Predict reactive species |
| Full Name | mitochondrial ribosomal protein L49 |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 19 kDa |
| GenBank Accession Number | BC004378 |
| Gene Symbol | MRPL49 |
| Gene ID (NCBI) | 740 |
| RRID | AB_2146189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MRPL49 antibody 15542-1-AP | Download protocol |
| IP protocol for MRPL49 antibody 15542-1-AP | Download protocol |
| WB protocol for MRPL49 antibody 15542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPL49 Polyclonal antibody (Cat# 15542-1-AP) has 6 total citations. These appear in Molecular Cell, Nucleic Acids Research (3 papers), Cell Reports, and Scientific Reports. The research fields encompass mitochondrial biology, including mitoribosome composition, mtDNA replication, mitochondrial translation, and mammalian cell size control. All cited applications were Western blot in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB. | ||
Nucleic Acids Res Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication. | ||
Nucleic Acids Res C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation. | ||
Cell Rep MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. | ||
Sci Rep Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure. |
