MRPL49 Polyclonal antibody, PBS Only

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• MRPL49 Polyclonal antibody, PBS Only (15542-1-PBS)

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  WB analysis of BxPC-3 using 15542-1-AP (same clone as 15542-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 15542-1-AP (same clone as 15542-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  IP result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

MRPL49 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15542-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

C11orf4, Large ribosomal subunit protein mL49, MRP-L49, NOF, NOF1

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Product Information

15542-1-PBS targets MRPL49 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7898

  Product name: Recombinant human MRPL49 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-166 aa of BC004378

  Sequence: MAATMFRATLRGWRTGVQRGCGLRLLSQTQGPPDYPRFVESVDEYQFVERLLPATRIPDPPKHEHYPTPSGWQPPRDPPPNLPYFVRRSRMHNIPVYKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLEKGF

• Full Name: mitochondrial ribosomal protein L49
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC004378
• Gene Symbol: MRPL49
• Gene ID (NCBI): 740
• RRID: AB_2146189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13405
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.