MRPL51 Recombinant monoclonal antibody, PBS Only

Name: MRPL51 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 83565-1-PBS-100UG

MRPL51 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83565-1-PBS

Clone No.240381C3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

240381C3, bMRP 64, bMRP64, bMRP-64, CDA09

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Proteintech Guarantee

Filter: AllWBIHCIF/ICCAffinity

WB analysis using 83565-1-RR (same clone as 83565-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83565-1-RR (MRPL51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)
WB result of MRPL51 antibody (83565-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL51 transfected HEK-293 cells. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovarian cancer using 83565-1-RR (same clone as 83565-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MRPL51 antibody (83565-1-RR, Clone: 240381C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 83565-1-RR
Biolayer interferometry (BLl) kinetic assays of 83565-1-RR against Human MRPL51 were performed. The affinity constant is 0.57 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83565-1-PBS targets MRPL51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag7193 Product name: Recombinant human MRPL51 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC000191 Sequence: MAGNLLSGAGRRLWDWVPLACRSFSLGVPRLIGIRLTLPPPKVVDRWNEKRAMFGVYDNIGILGNFEKHPKELIRGPIWLRGWKGNELQRCIRKRKMVGSRMFADDLHNLNKRIRYLYKHFNRHGKFR Predict reactive species
Full Name	mitochondrial ribosomal protein L51
Calculated Molecular Weight	15 kDa
Observed Molecular Weight	12 kDa
GenBank Accession Number	BC000191
Gene Symbol	MRPL51
Gene ID (NCBI)	51258
RRID	AB_3671180
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q4U2R6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Mitochondrial ribosome protein L51 (MRPL51) is a 39S subunit protein of the mitochondrial ribosome. MRPL51 functions in the translation of genetic messages (PMID: 21730110). MRPL51 expression was positively correlated with cell cycle, DNA damage, DNA repair, epithelial-mesenchymal transition, invasion, and proliferation of LUAD cells at the single cell level (PMID: 37323822). The calculated molecular weight of MRPL51 is 15 kDa.