MRPS23 Polyclonal antibody

MRPS23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18345-1-AP

Synonyms

MRPS 23, MRP S23, HSPC329, CGI-138, CGI138

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Validation Data Gallery

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  WB analysis using 18345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18345-1-AP (MRPS23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18345-1-AP

  IP result of anti-MRPS23 (IP:18345-1-AP, 4ug; Detection:18345-1-AP 1:300) with HeLa cells lysate 2600ug.

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  IHC staining of human liver cancer using 18345-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18345-1-AP (MRPS23 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18345-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18345-1-AP (MRPS23 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18345-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS23 antibody (18345-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

18345-1-AP targets MRPS23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS23 fusion protein Ag13144
• Full Name: mitochondrial ribosomal protein S23
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC000242
• Gene Symbol: MRPS23
• Gene ID (NCBI): 51649
• RRID: AB_2301078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
human	WB	Science; Autoregulatory control of mitochondrial glutathione homeostasis; Authors - Yuyang Liu
human	WB	Heliyon; LncRNA HIF1A-AS2 promotes triple-negative breast cancer progression and paclitaxel resistance via MRPS23 protein; Authors - Liangliang Min ; KD Validated