MRPS23 Polyclonal antibody

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• MRPS23 Polyclonal antibody (18345-1-AP)

MRPS23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18345-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MRPS 23, MRP S23, HSPC329, CGI-138, CGI138

• 

  WB analysis using 18345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18345-1-AP (MRPS23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 18345-1-AP

  IP result of anti-MRPS23 (IP:18345-1-AP, 4ug; Detection:18345-1-AP 1:300) with HeLa cells lysate 2600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 18345-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18345-1-AP (MRPS23 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 18345-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18345-1-AP (MRPS23 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 18345-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS23 antibody (18345-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18345-1-AP targets MRPS23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13144

  Product name: Recombinant human MRPS23 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-190 aa of BC000242

  Sequence: MAGSRLETVGSIFSRTRDLVRAGVLKEKPLWFDVYDAFPPLREPVFQRPRVRYGKAKAPIQDIWYHEDRIRAKFYSVYGSGQRAFDLFNPNFKSTCQRFVEKYTELQKLGETDEEKLFVETGKALLAEGVILRRVGEARTQHGGSHVSRKSEHLSVRPQTALEENETQKEVPQDQHLEAPADQSKGLLPP

• Full Name: mitochondrial ribosomal protein S23
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC000242
• Gene Symbol: MRPS23
• Gene ID (NCBI): 51649
• RRID: AB_2301078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y3D9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The MRPS23 Polyclonal antibody (Cat# 18345-1-AP) has 8 citations published in journals including Science, Signal Transduction and Targeted Therapy, Nature Structural & Molecular Biology, Nature Metabolism, International Journal of Biological Sciences, iScience, Heliyon, and bioRxiv. Research fields span mitochondrial biology, oxidative phosphorylation, ribosome assembly, ferroptosis/cell death, and oncology (nasopharyngeal carcinoma, liver cancer, triple-negative breast cancer).

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes; Authors - Wenyuan Wu
human	WB	Science; Autoregulatory control of mitochondrial glutathione homeostasis; Authors - Yuyang Liu
human	WB,IF	Int J Biol Sci; Co-targeting MRPS7-23 synergistically enhances cisplatin efficacy to suppress nasopharyngeal carcinoma growth and metastasis.; Authors - Zhangqi Cao
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia
human	WB	iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
		bioRxiv; Tumor Genotype Dictates Mitochondrial and Immune Vulnerabilities in Liver Cancer; Authors - Gokhan Unlu